A Brisbane-bound freight train containing food and beer derailed near the Australian Rail Track Corporation (ARTC) workshop on Sloane Street on Sunday.
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About 4.15am, five wagons went off the tracks, preventing any passage of trains into or out of Goulburn.
"Repair and recovery works have begun," the ARTC spokesperson said.
"No trains will run through Goulburn until recovery and rerailing works are completed."
A Goulburn Station worker said train services had been replaced by road coaches, meantime.
The ARTC was not able to confirm when normal services would return, but would provide updates throughout.